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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- MAB9502 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat iNOS Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P35228- Isotype
- IgG
- Antibody clone number
- 2D2-B2
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery.
Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns.
Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction.
High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy.
Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells.
Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide.
Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
Attenuation of apoptosis and enhancement of proteoglycan synthesis in rabbit cartilage defects by hyperbaric oxygen treatment are related to the suppression of nitric oxide production.
Ren W, Hou J, Yang C, Wang H, Wu S, Wu Y, Zhao X, Lu C
Journal of experimental & clinical cancer research : CR 2019 Feb 8;38(1):62
Journal of experimental & clinical cancer research : CR 2019 Feb 8;38(1):62
Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns.
Altan S, Oğurtan Z
Burns : journal of the International Society for Burn Injuries 2017 Feb;43(1):232-244
Burns : journal of the International Society for Burn Injuries 2017 Feb;43(1):232-244
Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction.
Yasuda Y, Fujita M, Koike E, Obata K, Shiota M, Kotani Y, Musha T, Tsuji-Kawahara S, Satou T, Masuda S, Okano J, Yamasaki H, Okumoto K, Uesugi T, Nakao S, Hoshiai H, Mandai M
PloS one 2015;10(4):e0122458
PloS one 2015;10(4):e0122458
High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy.
Dietrich S, Okun JG, Schmidt K, Falk CS, Wagner AH, Karamustafa S, Radujkovic A, Hegenbart U, Ho AD, Dreger P, Luft T
Haematologica 2014 Mar;99(3):541-7
Haematologica 2014 Mar;99(3):541-7
Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells.
Xia N, Pautz A, Wollscheid U, Reifenberg G, Förstermann U, Li H
Molecules (Basel, Switzerland) 2014 Mar 24;19(3):3654-68
Molecules (Basel, Switzerland) 2014 Mar 24;19(3):3654-68
Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
Kellom M, Basselin M, Keleshian VL, Chen M, Rapoport SI, Rao JS
BMC neuroscience 2012 May 23;13:50
BMC neuroscience 2012 May 23;13:50
The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide.
Espinosa A, Oke V, Elfving A, Nyberg F, Covacu R, Wahren-Herlenius M
Experimental cell research 2008 Dec 10;314(20):3605-13
Experimental cell research 2008 Dec 10;314(20):3605-13
Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM
Infection and immunity 2006 Dec;74(12):7010-3
Infection and immunity 2006 Dec;74(12):7010-3
Attenuation of apoptosis and enhancement of proteoglycan synthesis in rabbit cartilage defects by hyperbaric oxygen treatment are related to the suppression of nitric oxide production.
Yuan LJ, Ueng SW, Lin SS, Yeh WL, Yang CY, Lin PY
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2004 Sep;22(5):1126-34
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2004 Sep;22(5):1126-34
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human iNOS by Western Blot. Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta , Recombinant Human TNF-alpha , and Recombinant Human IFN-gamma (Catalog #201-LB, 210-TA, 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat iNOS by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iNOS at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse iNOS by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS, loaded at 0.2 mg/mL. A specific band was detected for iNOS at approximately 136 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- iNOS in Human Brain. iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
