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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [49]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB300-605 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-605, RRID:AB_10002794
- Product name
- Rabbit Polyclonal iNOS Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified. This antibody detects iNOS. It does not detect other NOS isoforms.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200uL
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration.
Therapeutic treatment with vitamin C reduces focal cerebral ischemia-induced brain infarction in rats by attenuating disruptions of blood brain barrier and cerebral neuronal apoptosis.
Dietary Arginine Regulates Severity of Experimental Colitis and Affects the Colonic Microbiome.
miR-511-3p protects against cockroach allergen-induced lung inflammation by antagonizing CCL2.
Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT-1-TREM-1 pathway in diabetic nephropathy.
Inducible nitric oxide synthase-derived nitric oxide reduces vagal satiety signalling in obese mice.
Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury by targeting MD2.
Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury.
C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis.
Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf-κβ activation in blunt pulmonary contusion model.
Semaphorin 4A acts in a feed-forward loop with NF-κB pathway to exacerbate catabolic effect of IL-1β on chondrocytes.
Targeting the phosphorylation site of myristoylated alanine-rich C kinase substrate alleviates symptoms in a murine model of steroid-resistant asthma.
ATF3 Stimulates IL-17A by Regulating Intracellular Ca(2+)/ROS-Dependent IL-1β Activation During Streptococcus pneumoniae Infection.
Downregulation of iNOS, IL-1β, and P2X7 Expression in Mast Cells via Activation of PAR4 Contributes to the Inhibition of Visceral Hyperalgesia in Rats.
Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway.
Zinc-Modified Sulfonated Polyetheretherketone Surface with Immunomodulatory Function for Guiding Cell Fate and Bone Regeneration.
Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo.
Heparanase regulates the M1 polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury.
An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation.
K284-6111 prevents the amyloid beta-induced neuroinflammation and impairment of recognition memory through inhibition of NF-κB-mediated CHI3L1 expression.
Inducible nitric oxide synthase-mediated injury in a mouse model of acute salivary gland dysfunction.
The Effects of Memantine on Glutamic Receptor-Associated Nitrosative Stress in a Traumatic Brain Injury Rat Model.
Augmented TME O-GlcNAcylation Promotes Tumor Proliferation through the Inhibition of p38 MAPK.
Tumor-Associated Macrophages and Regulatory T Cells Infiltration and the Clinical Outcome in Colorectal Cancer.
Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment.
Flavones modulate respiratory epithelial innate immunity: Anti-inflammatory effects and activation of the T2R14 receptor.
Inhibitory effect of punicalagin on lipopolysaccharide-induced neuroinflammation, oxidative stress and memory impairment via inhibition of nuclear factor-kappaB.
CC-chemokine class inhibition attenuates pathological angiogenesis while preserving physiological angiogenesis.
(E)-2-Methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates LPS-Mediated Memory Impairment by Inhibition of STAT3 Pathway.
Gastrodin Protects Neural Progenitor Cells Against Amyloid β (1-42)-Induced Neurotoxicity and Improves Hippocampal Neurogenesis in Amyloid β (1-42)-Injected Mice.
Tollip, an early regulator of the acute inflammatory response in the substantia nigra.
Wall stretch and thromboxane A₂ activate NO synthase (eNOS) in pulmonary arterial smooth muscle cells via H₂O₂ and Akt-dependent phosphorylation.
Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response.
Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps.
Myelin oligodendrocyte glycoprotein (MOG35-55)-induced experimental autoimmune encephalomyelitis is ameliorated in interleukin-32 alpha transgenic mice.
Toward Repositioning Niclosamide for Antivirulence Therapy of Pseudomonas aeruginosa Lung Infections: Development of Inhalable Formulations through Nanosuspension Technology.
LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14.
Hypoxia-augmented constriction of deep femoral artery mediated by inhibition of eNOS in smooth muscle.
Involvement of extraneural tissues and upregulation of inducible nitric oxide synthase after experimental infection with rabies virus in BALB/c mice and LEW/SsN rats.
Phosphoinositide 3-kinase in nitric oxide synthesis in macrophage: critical dimerization of inducible nitric-oxide synthase.
Activated macrophages are essential in a murine model for T cell-mediated chronic psoriasiform skin inflammation.
Endocardial endothelium in the avascular frog heart: role for diffusion of NO in control of cardiac O2 consumption.
Myocyte nitric oxide synthase 2 contributes to blunted beta-adrenergic response in failing human hearts by decreasing Ca2+ transients.
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension.
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension.
Differential inducible nitric oxide synthase expression in systemic and pulmonary vessels after endotoxin.
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
Saclier M, Lapi M, Bonfanti C, Rossi G, Antonini S, Messina G
Cells 2020 Mar 13;9(3)
Cells 2020 Mar 13;9(3)
Therapeutic treatment with vitamin C reduces focal cerebral ischemia-induced brain infarction in rats by attenuating disruptions of blood brain barrier and cerebral neuronal apoptosis.
Chang CY, Chen JY, Wu MH, Hu ML
Free radical biology & medicine 2020 Aug 1;155:29-36
Free radical biology & medicine 2020 Aug 1;155:29-36
Dietary Arginine Regulates Severity of Experimental Colitis and Affects the Colonic Microbiome.
Singh K, Gobert AP, Coburn LA, Barry DP, Allaman M, Asim M, Luis PB, Schneider C, Milne GL, Boone HH, Shilts MH, Washington MK, Das SR, Piazuelo MB, Wilson KT
Frontiers in cellular and infection microbiology 2019;9:66
Frontiers in cellular and infection microbiology 2019;9:66
miR-511-3p protects against cockroach allergen-induced lung inflammation by antagonizing CCL2.
Do DC, Mu J, Ke X, Sachdeva K, Qin Z, Wan M, Ishmael FT, Gao P
JCI insight 2019 Oct 17;4(20)
JCI insight 2019 Oct 17;4(20)
Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT-1-TREM-1 pathway in diabetic nephropathy.
Zhang X, Zhao Y, Zhu X, Guo Y, Yang Y, Jiang Y, Liu B
Journal of cellular physiology 2019 May;234(5):6917-6926
Journal of cellular physiology 2019 May;234(5):6917-6926
Inducible nitric oxide synthase-derived nitric oxide reduces vagal satiety signalling in obese mice.
Yu Y, Park SJ, Beyak MJ
The Journal of physiology 2019 Mar;597(6):1487-1502
The Journal of physiology 2019 Mar;597(6):1487-1502
Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury by targeting MD2.
Ye J, Guan M, Lu Y, Zhang D, Li C, Li Y, Zhou C
European journal of pharmacology 2019 Jun 5;852:151-158
European journal of pharmacology 2019 Jun 5;852:151-158
Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury.
Marek-Iannucci S, Thomas A, Hou J, Crupi A, Sin J, Taylor DJ, Czer LS, Esmailian F, Mentzer RM Jr, Andres AM, Gottlieb RA
Scientific reports 2019 Jul 10;9(1):10001
Scientific reports 2019 Jul 10;9(1):10001
C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis.
Shao Q, Yang M, Liang C, Ma L, Zhang W, Jiang Z, Luo J, Lee JK, Liang C, Chen JF
Autophagy 2019 Dec 26;:1-16
Autophagy 2019 Dec 26;:1-16
Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf-κβ activation in blunt pulmonary contusion model.
Karaboğa İ
Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES 2019 Aug;25(5):433-439
Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES 2019 Aug;25(5):433-439
Semaphorin 4A acts in a feed-forward loop with NF-κB pathway to exacerbate catabolic effect of IL-1β on chondrocytes.
Zhang H, Wei Q, Xiang X, Zhou B, Chen J, Li J, Li Q, Xiong H, Liu F
International immunopharmacology 2019 Apr;69:88-94
International immunopharmacology 2019 Apr;69:88-94
Targeting the phosphorylation site of myristoylated alanine-rich C kinase substrate alleviates symptoms in a murine model of steroid-resistant asthma.
Wang CN, Lin YC, Chang BC, Chen CH, Wu R, Lee CC
British journal of pharmacology 2019 Apr;176(8):1122-1134
British journal of pharmacology 2019 Apr;176(8):1122-1134
ATF3 Stimulates IL-17A by Regulating Intracellular Ca(2+)/ROS-Dependent IL-1β Activation During Streptococcus pneumoniae Infection.
Lee S, Kim GL, Kim NY, Kim SJ, Ghosh P, Rhee DK
Frontiers in immunology 2018;9:1954
Frontiers in immunology 2018;9:1954
Downregulation of iNOS, IL-1β, and P2X7 Expression in Mast Cells via Activation of PAR4 Contributes to the Inhibition of Visceral Hyperalgesia in Rats.
Hao Y, Niu H, An S, Wang M, Wang Z
Journal of immunology research 2018;2018:3256908
Journal of immunology research 2018;2018:3256908
Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway.
Gu SM, Lee HP, Ham YW, Son DJ, Kim HY, Oh KW, Han SB, Yun J, Hong JT
Neuromolecular medicine 2018 Sep;20(3):312-327
Neuromolecular medicine 2018 Sep;20(3):312-327
Zinc-Modified Sulfonated Polyetheretherketone Surface with Immunomodulatory Function for Guiding Cell Fate and Bone Regeneration.
Liu W, Li J, Cheng M, Wang Q, Yeung KWK, Chu PK, Zhang X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2018 Oct;5(10):1800749
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2018 Oct;5(10):1800749
Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo.
Shaalan A, Carpenter G, Proctor G
Molecular immunology 2018 Feb;94:190-199
Molecular immunology 2018 Feb;94:190-199
Heparanase regulates the M1 polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury.
Masola V, Zaza G, Bellin G, Dall'Olmo L, Granata S, Vischini G, Secchi MF, Lupo A, Gambaro G, Onisto M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Feb;32(2):742-756
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Feb;32(2):742-756
An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation.
Zhu J, He J, Verano M, Brimmo AT, Glia A, Qasaimeh MA, Chen P, Aleman JO, Chen W
Lab on a chip 2018 Dec 7;18(23):3550-3560
Lab on a chip 2018 Dec 7;18(23):3550-3560
K284-6111 prevents the amyloid beta-induced neuroinflammation and impairment of recognition memory through inhibition of NF-κB-mediated CHI3L1 expression.
Choi JY, Yeo IJ, Kim KC, Choi WR, Jung JK, Han SB, Hong JT
Journal of neuroinflammation 2018 Aug 11;15(1):224
Journal of neuroinflammation 2018 Aug 11;15(1):224
Inducible nitric oxide synthase-mediated injury in a mouse model of acute salivary gland dysfunction.
Shaalan A, Carpenter G, Proctor G
Nitric oxide : biology and chemistry 2018 Aug 1;78:95-102
Nitric oxide : biology and chemistry 2018 Aug 1;78:95-102
The Effects of Memantine on Glutamic Receptor-Associated Nitrosative Stress in a Traumatic Brain Injury Rat Model.
Wang CC, Wee HY, Hu CY, Chio CC, Kuo JR
World neurosurgery 2018 Apr;112:e719-e731
World neurosurgery 2018 Apr;112:e719-e731
Augmented TME O-GlcNAcylation Promotes Tumor Proliferation through the Inhibition of p38 MAPK.
Moriwaki K, Asahi M
Molecular cancer research : MCR 2017 Sep;15(9):1287-1298
Molecular cancer research : MCR 2017 Sep;15(9):1287-1298
Tumor-Associated Macrophages and Regulatory T Cells Infiltration and the Clinical Outcome in Colorectal Cancer.
Waniczek D, Lorenc Z, Śnietura M, Wesecki M, Kopec A, Muc-Wierzgoń M
Archivum immunologiae et therapiae experimentalis 2017 Oct;65(5):445-454
Archivum immunologiae et therapiae experimentalis 2017 Oct;65(5):445-454
Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment.
Choi JY, Jang JS, Son DJ, Im HS, Kim JY, Park JE, Choi WR, Han SB, Hong JT
International journal of molecular sciences 2017 Nov 28;18(12)
International journal of molecular sciences 2017 Nov 28;18(12)
Flavones modulate respiratory epithelial innate immunity: Anti-inflammatory effects and activation of the T2R14 receptor.
Hariri BM, McMahon DB, Chen B, Freund JR, Mansfield CJ, Doghramji LJ, Adappa ND, Palmer JN, Kennedy DW, Reed DR, Jiang P, Lee RJ
The Journal of biological chemistry 2017 May 19;292(20):8484-8497
The Journal of biological chemistry 2017 May 19;292(20):8484-8497
Inhibitory effect of punicalagin on lipopolysaccharide-induced neuroinflammation, oxidative stress and memory impairment via inhibition of nuclear factor-kappaB.
Kim YE, Hwang CJ, Lee HP, Kim CS, Son DJ, Ham YW, Hellström M, Han SB, Kim HS, Park EK, Hong JT
Neuropharmacology 2017 May 1;117:21-32
Neuropharmacology 2017 May 1;117:21-32
CC-chemokine class inhibition attenuates pathological angiogenesis while preserving physiological angiogenesis.
Ridiandries A, Tan JT, Ravindran D, Williams H, Medbury HJ, Lindsay L, Hawkins C, Prosser HC, Bursill CA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1179-1192
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1179-1192
(E)-2-Methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates LPS-Mediated Memory Impairment by Inhibition of STAT3 Pathway.
Choi JY, Hwang CJ, Lee DY, Gu SM, Lee HP, Choi DY, Oh KW, Han SB, Hong JT
Neuromolecular medicine 2017 Dec;19(4):555-570
Neuromolecular medicine 2017 Dec;19(4):555-570
Gastrodin Protects Neural Progenitor Cells Against Amyloid β (1-42)-Induced Neurotoxicity and Improves Hippocampal Neurogenesis in Amyloid β (1-42)-Injected Mice.
Li M, Qian S
Journal of molecular neuroscience : MN 2016 Sep;60(1):21-32
Journal of molecular neuroscience : MN 2016 Sep;60(1):21-32
Tollip, an early regulator of the acute inflammatory response in the substantia nigra.
Humbert-Claude M, Duc D, Dwir D, Thieren L, Sandström von Tobel J, Begka C, Legueux F, Velin D, Maillard MH, Do KQ, Monnet-Tschudi F, Tenenbaum L
Journal of neuroinflammation 2016 Dec 7;13(1):303
Journal of neuroinflammation 2016 Dec 7;13(1):303
Wall stretch and thromboxane A₂ activate NO synthase (eNOS) in pulmonary arterial smooth muscle cells via H₂O₂ and Akt-dependent phosphorylation.
Kim HJ, Yoo HY, Jang JH, Lin HY, Seo EY, Zhang YH, Kim SJ
Pflugers Archiv : European journal of physiology 2016 Apr;468(4):705-16
Pflugers Archiv : European journal of physiology 2016 Apr;468(4):705-16
Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response.
Wezel A, de Vries MR, Maassen JM, Kip P, Peters EA, Karper JC, Kuiper J, Bot I, Quax PHA
Scientific reports 2016 Apr 7;6:24248
Scientific reports 2016 Apr 7;6:24248
Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps.
Kober C, Rohn S, Weibel S, Geissinger U, Chen NG, Szalay AA
Journal of translational medicine 2015 Jul 7;13:216
Journal of translational medicine 2015 Jul 7;13:216
Myelin oligodendrocyte glycoprotein (MOG35-55)-induced experimental autoimmune encephalomyelitis is ameliorated in interleukin-32 alpha transgenic mice.
Yun J, Gu SM, Yun HM, Son DJ, Park MH, Lee MS, Hong JT
Oncotarget 2015 Dec 1;6(38):40452-63
Oncotarget 2015 Dec 1;6(38):40452-63
Toward Repositioning Niclosamide for Antivirulence Therapy of Pseudomonas aeruginosa Lung Infections: Development of Inhalable Formulations through Nanosuspension Technology.
Costabile G, d'Angelo I, Rampioni G, Bondì R, Pompili B, Ascenzioni F, Mitidieri E, d'Emmanuele di Villa Bianca R, Sorrentino R, Miro A, Quaglia F, Imperi F, Leoni L, Ungaro F
Molecular pharmaceutics 2015 Aug 3;12(8):2604-17
Molecular pharmaceutics 2015 Aug 3;12(8):2604-17
LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14.
Tarassishin L, Suh HS, Lee SC
Glia 2014 Jun;62(6):999-1013
Glia 2014 Jun;62(6):999-1013
Hypoxia-augmented constriction of deep femoral artery mediated by inhibition of eNOS in smooth muscle.
Han JA, Seo EY, Kim HJ, Park SJ, Yoo HY, Kim JY, Shin DM, Kim JK, Zhang YH, Kim SJ
American journal of physiology. Cell physiology 2013 Jan 1;304(1):C78-88
American journal of physiology. Cell physiology 2013 Jan 1;304(1):C78-88
Involvement of extraneural tissues and upregulation of inducible nitric oxide synthase after experimental infection with rabies virus in BALB/c mice and LEW/SsN rats.
Liao PH, Hsu YH, Yang HH, Wang MH, Chen LK
Pathology international 2012 Sep;62(9):619-27
Pathology international 2012 Sep;62(9):619-27
Phosphoinositide 3-kinase in nitric oxide synthesis in macrophage: critical dimerization of inducible nitric-oxide synthase.
Sakai K, Suzuki H, Oda H, Akaike T, Azuma Y, Murakami T, Sugi K, Ito T, Ichinose H, Koyasu S, Shirai M
The Journal of biological chemistry 2006 Jun 30;281(26):17736-42
The Journal of biological chemistry 2006 Jun 30;281(26):17736-42
Activated macrophages are essential in a murine model for T cell-mediated chronic psoriasiform skin inflammation.
Wang H, Peters T, Kess D, Sindrilaru A, Oreshkova T, Van Rooijen N, Stratis A, Renkl AC, Sunderkötter C, Wlaschek M, Haase I, Scharffetter-Kochanek K
The Journal of clinical investigation 2006 Aug;116(8):2105-14
The Journal of clinical investigation 2006 Aug;116(8):2105-14
Endocardial endothelium in the avascular frog heart: role for diffusion of NO in control of cardiac O2 consumption.
Adler A, Huang H, Wang Z, Conetta J, Levee E, Zhang X, Hintze TH
American journal of physiology. Heart and circulatory physiology 2004 Jul;287(1):H14-21
American journal of physiology. Heart and circulatory physiology 2004 Jul;287(1):H14-21
Myocyte nitric oxide synthase 2 contributes to blunted beta-adrenergic response in failing human hearts by decreasing Ca2+ transients.
Ziolo MT, Maier LS, Piacentino V 3rd, Bossuyt J, Houser SR, Bers DM
Circulation 2004 Apr 20;109(15):1886-91
Circulation 2004 Apr 20;109(15):1886-91
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension.
Fagan KA, Morrissey B, Fouty BW, Sato K, Harral JW, Morris KG Jr, Hoedt-Miller M, Vidmar S, McMurtry IF, Rodman DM
Respiratory research 2001;2(5):306-13
Respiratory research 2001;2(5):306-13
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension.
Fagan KA, Morrissey B, Fouty BW, Sato K, Harral JW, Morris KG Jr, Hoedt-Miller M, Vidmar S, McMurtry IF, Rodman DM
Respiratory research 2001;2(5):306-13
Respiratory research 2001;2(5):306-13
Differential inducible nitric oxide synthase expression in systemic and pulmonary vessels after endotoxin.
Pulido EJ, Shames BD, Fullerton DA, Sheridan BC, Selzman CH, Gamboni-Robertson F, Bensard DD, McIntyre RC Jr
American journal of physiology. Regulatory, integrative and comparative physiology 2000 May;278(5):R1232-9
American journal of physiology. Regulatory, integrative and comparative physiology 2000 May;278(5):R1232-9
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
Xu KY, Huso DL, Dawson TM, Bredt DS, Becker LC
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
Xu KY, Huso DL, Dawson TM, Bredt DS, Becker LC
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
Proceedings of the National Academy of Sciences of the United States of America 1999 Jan 19;96(2):657-62
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
Bredt DS, Hwang PM, Glatt CE, Lowenstein C, Reed RR, Snyder SH
Nature 1991 Jun 27;351(6329):714-8
Nature 1991 Jun 27;351(6329):714-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
