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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN953738 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 837-868), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 837-868aa) of human NOS2A
- Description
- Purified through a Protein A column followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 837-868,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references [The role of polymorphic variants of gene of inducible NO-synthase NOS2 in brain infarction in patients with acute ischemic stroke].
The (CCTTT)n microsatellite polymorphism in the nitric oxide synthase 2 gene may influence bladder cancer pathogenesis.
Inducible nitric oxide synthase in duodenum of children with Giardia lamblia infection.
Tupitsyna TV, Bondarenko EA, Botsina AIu, Shetova IM, Limborskaia SA, Skvortsova VI, Slominskiĭ PA
Molekuliarnaia genetika, mikrobiologiia i virusologiia 2010;(3):3-7
Molekuliarnaia genetika, mikrobiologiia i virusologiia 2010;(3):3-7
The (CCTTT)n microsatellite polymorphism in the nitric oxide synthase 2 gene may influence bladder cancer pathogenesis.
Ryk C, Steineck G, Wiklund NP, Nyberg T, de Verdier PJ
The Journal of urology 2010 Nov;184(5):2150-7
The Journal of urology 2010 Nov;184(5):2150-7
Inducible nitric oxide synthase in duodenum of children with Giardia lamblia infection.
Mokrzycka M, Kolasa A, Kosierkiewicz A, Wiszniewska B
Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society 2010 Jan;48(2):191-6
Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society 2010 Jan;48(2):191-6
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis in CEM cell line lysates (35ug/lane) using NOS2 antibody Cat.-No. AP52914PU-N. This demonstrates this antibody detected the NOS2 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NOS2 with hela cells using NOS2 antibody Cat.-No. AP52914PU-N, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis human hepato- carcinoma (Formalin-fixed, Paraffin-embedded) using NOS2 antibody Cat.-No. AP52914PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram) using NOS2 antibody Cat.-No. AP52914PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
