Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [6]
- Immunohistochemistry [5]
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- Product number
- PA5-96256 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX6A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SSGAHGEEGS ARMWKTLTFF VALPGVAVSM LNVYLKSHHG EHERPEFIAY PHLRIRTKPF PWGDGNHTLF HNPHVNPLPT GYEDE; Positive Samples: A-549, MCF7, Mouse kidney, Mouse heart, Mouse liver, Rat liver, Rat brain; Cellular Location: Mitochondrion inner membrane
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.79 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX6A1 in extracts of various cell lines. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX6A1 was performed in C6 cells using COX6A1 Polyclonal Antibody (Product # PA5-96256) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX6A1 was performed in NIH-3T3 cells using COX6A1 Polyclonal Antibody (Product # PA5-96256) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX6A1 was performed in U-2 OS cells using COX6A1 Polyclonal Antibody (Product # PA5-96256) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of COX6A1 in C6 cells. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of COX6A1 in NIH-3T3 cells. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of COX6A1 in U-2 OS cells. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX6A1 in paraffin-embedded human liver cancer. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX6A1 in paraffin-embedded rat cerebellum. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX6A1 in paraffin-embedded mouse liver. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX6A1 in paraffin-embedded rat cerebellum. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX6A1 in paraffin-embedded human liver cancer. Samples were incubated with COX6A1 Polyclonal antibody (Product # PA5-96256) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.