MA5-50293

antibody from Invitrogen Antibodies

Targeting: SLN MGC12301, MGC125854, MGC125855

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50293

Provider

Invitrogen Antibodies

Product name

SLN Monoclonal Antibody (14G1A12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

14G1A12

Vial size

100 µL

Concentration

1.13 mg/mL

Storage

-20°C or -80°C if preferred

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis with a SLN monoclonal antibody (Product # MA5-50293) using a dilution of 1:60 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis overlay peak curve showing U87 cells stained with SLN monoclonal antibody (Product # MA5-50293) (red line) at 1:60. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1 µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1 µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.