Antibody data
- Antibody Data
- Antigen structure
- References [35]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- 10832-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10832-1-AP, RRID:AB_2065392
- Product name
- BMI1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated BMI1 antibody (Cat. #10832-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Celastrol mediates CAV1 to attenuate pro-tumorigenic effects of senescent cells.
Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC.
SEC61 translocon subunit gamma enhances low-dose cisplatin-induced cancer-stem cell properties of head and neck squamous cell carcinoma via enhancing Ca(2+)-mediated autophagy.
Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma.
Targeting PCSK9 reduces cancer cell stemness and enhances antitumor immunity in head and neck cancer.
BMI-1 promotes breast cancer proliferation and metastasis through different mechanisms in different subtypes.
Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy.
SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions.
A Sonic Hedgehog-Gli-Bmi1 signaling pathway plays a critical role in p27 deficiency induced bone anabolism.
BMI1 governs the maintenance of mouse GC-2 cells through epigenetic repression of Foxl1 transcription.
Identification of proline-rich protein 11 as a major regulator in mouse spermatogonia maintenance via an increase in BMI1 protein stability.
PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c-Myc-mediated positive feedback loop.
MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling.
FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.
Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.
miR-135a Reduces Osteosarcoma Pulmonary Metastasis by Targeting Both BMI1 and KLF4.
A further study on a disturbance of intestinal epithelial cell population and kinetics in APC1638T mice.
FOXD1 promotes EMT and cell stemness of oral squamous cell carcinoma by transcriptional activation of SNAI2.
CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β‑amyloid metabolism‑associated enzymes.
Internal Ribosome Entry Sites Mediate Cap-Independent Translation of Bmi1 in Nasopharyngeal Carcinoma.
CCL21/CCR7 interaction promotes EMT and enhances the stemness of OSCC via a JAK2/STAT3 signaling pathway.
UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.
ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
Chlorogenic acid inhibits esophageal squamous cell carcinoma growth in vitro and in vivo by downregulating the expression of BMI1 and SOX2.
Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.
R-spondin1/Wnt-enhanced Ascl2 autoregulation controls the self-renewal of colorectal cancer progenitor cells.
Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease.
Downregulation of MiR-203a Disinhibits Bmi1 and Promotes Growth and Proliferation of Keratinocytes in Cholesteatoma.
SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.
A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.
Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.
Preclinical activity of the novel B-cell-specific Moloney murine leukemia virus integration site 1 inhibitor PTC-209 in acute myeloid leukemia: Implications for leukemia therapy.
Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.
Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.
Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.
Zhang S, Zhu N, Shi YN, Zeng Q, Zhang CJ, Li HF, Qin L
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul;129:155614
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul;129:155614
Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC.
Zhao X, Guo B, Sun W, Yu J, Cui L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Sep;10(27):e2206878
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Sep;10(27):e2206878
SEC61 translocon subunit gamma enhances low-dose cisplatin-induced cancer-stem cell properties of head and neck squamous cell carcinoma via enhancing Ca(2+)-mediated autophagy.
Bu X, Zhang J, Sun H, Wang W, Ma X, Sun L
Journal of dental sciences 2023 Oct;18(4):1685-1692
Journal of dental sciences 2023 Oct;18(4):1685-1692
Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma.
Fang Z, Kong F, Zeng J, Zhang Z, Wang Y, Wang Y, Duan J, Chen L, Wang J, Liu F
Cancer medicine 2023 Jun;12(11):12943-12959
Cancer medicine 2023 Jun;12(11):12943-12959
Targeting PCSK9 reduces cancer cell stemness and enhances antitumor immunity in head and neck cancer.
Yang QC, Wang S, Liu YT, Song A, Wu ZZ, Wan SC, Li HM, Sun ZJ
iScience 2023 Jun 16;26(6):106916
iScience 2023 Jun 16;26(6):106916
BMI-1 promotes breast cancer proliferation and metastasis through different mechanisms in different subtypes.
Liu JY, Jiang YN, Huang H, Xu JF, Wu YH, Wang Q, Zhu Y, Zheng B, Shen C, Qian WF, Shen J
Cancer science 2023 Feb;114(2):449-462
Cancer science 2023 Feb;114(2):449-462
Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy.
Jing B, Guo F, An R, Gao Y, Li Y, Xie Y, Wang J, Chen Y, Li H, Gao T, Jin Q, Zhang L, Xie M
Journal of nanobiotechnology 2023 Feb 2;21(1):37
Journal of nanobiotechnology 2023 Feb 2;21(1):37
SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions.
Zhou H, Feng W, Yu J, Shafiq TA, Paulo JA, Zhang J, Luo Z, Gygi SP, Moazed D
Cell reports 2023 Apr 25;42(4):112339
Cell reports 2023 Apr 25;42(4):112339
A Sonic Hedgehog-Gli-Bmi1 signaling pathway plays a critical role in p27 deficiency induced bone anabolism.
Wu J, Wang R, Kan X, Zhang J, Sun W, Goltzman D, Miao D
International journal of biological sciences 2022;18(3):956-969
International journal of biological sciences 2022;18(3):956-969
BMI1 governs the maintenance of mouse GC-2 cells through epigenetic repression of Foxl1 transcription.
Zheng B, Liu J, Shi X, Xu J, Zhang K, Zhou H, Wu T, Huang X, Shen C, Liang Y, Zhao D, Guo Y
American journal of translational research 2022;14(5):3407-3418
American journal of translational research 2022;14(5):3407-3418
Identification of proline-rich protein 11 as a major regulator in mouse spermatogonia maintenance via an increase in BMI1 protein stability.
Xue J, Wu T, Huang C, Shu M, Shen C, Zheng B, Lv J
Molecular biology reports 2022 Oct;49(10):9555-9564
Molecular biology reports 2022 Oct;49(10):9555-9564
PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c-Myc-mediated positive feedback loop.
Zhao X, Shu D, Sun W, Si S, Ran W, Guo B, Cui L
Cancer communications (London, England) 2022 Oct;42(10):987-1007
Cancer communications (London, England) 2022 Oct;42(10):987-1007
MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling.
Zhou X, Luo J, Xie H, Wei Z, Li T, Liu J, Liao X, Zhu G, Peng T
Cell death discovery 2022 Oct 15;8(1):418
Cell death discovery 2022 Oct 15;8(1):418
FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.
Zhao X, Chen H, Qiu Y, Cui L
International journal of oral science 2022 May 10;14(1):25
International journal of oral science 2022 May 10;14(1):25
Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.
Chen Y, Zhao H, Liang W, Jiang E, Zhou X, Shao Z, Liu K, Shang Z
Oncogene 2022 Jan;41(5):634-646
Oncogene 2022 Jan;41(5):634-646
miR-135a Reduces Osteosarcoma Pulmonary Metastasis by Targeting Both BMI1 and KLF4.
Chen C, Mao X, Cheng C, Jiao Y, Zhou Y, Ren T, Wu Z, Lv Z, Sun X, Guo W
Frontiers in oncology 2021;11:620295
Frontiers in oncology 2021;11:620295
A further study on a disturbance of intestinal epithelial cell population and kinetics in APC1638T mice.
Wenduerma, Yamada NO, Wang T, Senda T
Medical molecular morphology 2021 Sep;54(3):203-215
Medical molecular morphology 2021 Sep;54(3):203-215
FOXD1 promotes EMT and cell stemness of oral squamous cell carcinoma by transcriptional activation of SNAI2.
Chen Y, Liang W, Liu K, Shang Z
Cell & bioscience 2021 Aug 4;11(1):154
Cell & bioscience 2021 Aug 4;11(1):154
CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β‑amyloid metabolism‑associated enzymes.
Jiang H, Niu F, Zheng Y, Xu Y
Molecular medicine reports 2021 Apr;23(4)
Molecular medicine reports 2021 Apr;23(4)
Internal Ribosome Entry Sites Mediate Cap-Independent Translation of Bmi1 in Nasopharyngeal Carcinoma.
Wang H, Zhu Y, Hu L, Li Y, Liu G, Xia T, Xiong D, Luo Y, Liu B, An Y, Li M, Huang Y, Zhong Q, Zeng M
Frontiers in oncology 2020;10:1678
Frontiers in oncology 2020;10:1678
CCL21/CCR7 interaction promotes EMT and enhances the stemness of OSCC via a JAK2/STAT3 signaling pathway.
Chen Y, Shao Z, Jiang E, Zhou X, Wang L, Wang H, Luo X, Chen Q, Liu K, Shang Z
Journal of cellular physiology 2020 Sep;235(9):5995-6009
Journal of cellular physiology 2020 Sep;235(9):5995-6009
UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.
Sun J, Zhu Z, Li W, Shen M, Cao C, Sun Q, Guo Z, Liu L, Wu D
Journal of experimental & clinical cancer research : CR 2020 Oct 21;39(1):222
Journal of experimental & clinical cancer research : CR 2020 Oct 21;39(1):222
ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
Ran J, Liu M, Feng J, Li H, Ma H, Song T, Cao Y, Zhou P, Wu Y, Yang Y, Yang Y, Yu F, Guo H, Zhang L, Xie S, Li D, Gao J, Zhang X, Zhu X, Zhou J
Developmental cell 2020 May 4;53(3):287-299.e5
Developmental cell 2020 May 4;53(3):287-299.e5
Chlorogenic acid inhibits esophageal squamous cell carcinoma growth in vitro and in vivo by downregulating the expression of BMI1 and SOX2.
Zhan Y, Li R, Feng C, Li X, Huang S, Wang L, Liu Z, Jiang J, Han Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Jan;121:109602
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Jan;121:109602
Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.
Li C, Zheng H, Hou W, Bao H, Xiong J, Che W, Gu Y, Sun H, Liang P
Cell death & disease 2019 Sep 26;10(10):717
Cell death & disease 2019 Sep 26;10(10):717
R-spondin1/Wnt-enhanced Ascl2 autoregulation controls the self-renewal of colorectal cancer progenitor cells.
Ye J, Liu S, Shang Y, Chen H, Wang R
Cell cycle (Georgetown, Tex.) 2018;17(8):1014-1025
Cell cycle (Georgetown, Tex.) 2018;17(8):1014-1025
Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease.
Su Y, Wen Z, Shen Q, Zhang H, Peng L, Chen G, Zhu Z, Du C, Xie H, Li H, Xia Y, Tang W
Cell cycle (Georgetown, Tex.) 2018;17(4):459-467
Cell cycle (Georgetown, Tex.) 2018;17(4):459-467
Downregulation of MiR-203a Disinhibits Bmi1 and Promotes Growth and Proliferation of Keratinocytes in Cholesteatoma.
Zang J, Hui L, Yang N, Yang B, Jiang X
International journal of medical sciences 2018;15(5):447-455
International journal of medical sciences 2018;15(5):447-455
SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.
Xie SL, Fan S, Zhang SY, Chen WX, Li QX, Pan GK, Zhang HQ, Wang WW, Weng B, Zhang Z, Li JS, Lin ZY
International journal of cancer 2018 Mar 15;142(6):1252-1265
International journal of cancer 2018 Mar 15;142(6):1252-1265
A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.
Wang X, Cao W, Zhang J, Yan M, Xu Q, Wu X, Wan L, Zhang Z, Zhang C, Qin X, Xiao M, Ye D, Liu Y, Han Z, Wang S, Mao L, Wei W, Chen W
The EMBO journal 2017 May 2;36(9):1243-1260
The EMBO journal 2017 May 2;36(9):1243-1260
Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.
Wei X, Ye J, Shang Y, Chen H, Liu S, Liu L, Wang R
Oncotarget 2017 Dec 12;8(65):109301-109318
Oncotarget 2017 Dec 12;8(65):109301-109318
Preclinical activity of the novel B-cell-specific Moloney murine leukemia virus integration site 1 inhibitor PTC-209 in acute myeloid leukemia: Implications for leukemia therapy.
Nishida Y, Maeda A, Chachad D, Ishizawa J, Qiu YH, Kornblau SM, Kimura S, Andreeff M, Kojima K
Cancer science 2015 Dec;106(12):1705-13
Cancer science 2015 Dec;106(12):1705-13
Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.
Wang HB, Liu GH, Zhang H, Xing S, Hu LJ, Zhao WF, Xie B, Li MZ, Zeng BH, Li Y, Zeng MS
The FEBS journal 2013 Jun;280(12):2929-44
The FEBS journal 2013 Jun;280(12):2929-44
Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.
Wu Z, Min L, Chen D, Hao D, Duan Y, Qiu G, Wang Y
PloS one 2011 Feb 2;6(2):e14648
PloS one 2011 Feb 2;6(2):e14648
Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.
Kim MO, Chawla P, Overland RP, Xia E, Sadri-Vakili G, Cha JH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 9;28(15):3947-57
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 9;28(15):3947-57
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- U-937 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP(BMI1 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver cancer using 10832-1-AP(BMI1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue