PAB12958

antibody from Abnova Corporation

Targeting: BMI1 PCGF4, RNF51

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12958

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12958, RRID:AB_10546962

Product name

BMI1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of BMI1.

Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.

Lessard J, Sauvageau G
Nature 2003 May 15;423(6937):255-60

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The oncogene and Polycomb-group gene bmi-1 regulates cell proliferation and senescence through the ink4a locus.

Jacobs JJ, Kieboom K, Marino S, DePinho RA, van Lohuizen M
Nature 1999 Jan 14;397(6715):164-8

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Characterization and chromosomal localization of the human proto-oncogene BMI-1.

Alkema MJ, Wiegant J, Raap AK, Berns A, van Lohuizen M
Human molecular genetics 1993 Oct;2(10):1597-603

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of BMI1 in K-562 cell lysate with BMI1 polyclonal antibody (Cat # PAB12958) at (A) 0.5, (B) 1 and (C) 2 ug/mL .

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemistry of BMI1 in K-562 cells with BMI1 polyclonal antibody (Cat # PAB12958) at 10 ug/mL .

Validation comment

Immunocytochemistry