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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-15919 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMI-1 Monoclonal Antibody (3E3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15919 targets BMI1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15919 immunogen is purified recombinant fragment of human BMI1 expressed in E. Coli. . MA5-15919 detects BMI1 which has a predicted molecular weight of approximately 37kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E3
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references BMI1 maintains the Treg epigenomic landscape to prevent inflammatory bowel disease.
Gonzalez MM, Bamidele AO, Svingen PA, Sagstetter MR, Smyrk TC, Gaballa JM, Hamdan FH, Kosinsky RL, Gibbons HR, Sun Z, Ye Z, Nair A, Ramos GP, Braga Neto MB, Wixom AQ, Mathison AJ, Johnsen SA, Urrutia R, Faubion WA Jr
The Journal of clinical investigation 2021 Jun 15;131(12)
The Journal of clinical investigation 2021 Jun 15;131(12)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMI1 using BMI1 monoclonal antibody (Product # MA5-15919) in HEK293 (1) and BMI1-human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMI1 using a BMI1 monoclonal antibody (Product # MA5-15919) against a human BMI1 (AA: 1-326) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMI1 using BMI1 monoclonal antibody (Product # MA5-15919) in HEK293 (1) and BMI1-human IgG Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using BMI1 monoclonal antibody (Product # MA5-15919) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 monoclonal antibody (Product # MA5-15919) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 monoclonal antibody (Product # MA5-15919) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using BMI1 monoclonal antibody (Product # MA5-15919) (green) and negative control (red).
