A302-771A

antibody from Invitrogen Antibodies

Targeting: NPAT E14, p220

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-771A

Provider

Invitrogen Antibodies

Product name

NPAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NPAT by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPAT (Cat. No. A302-771A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NPAT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NPAT antibody A302-771A used for IP at 3 µg/mg lysate. NPAT was also immunoprecipitated by rabbit anti-NPAT antibody A302-772A, which recognizes a downstream epitope. For blotting immunoprecipitated NPAT, A302-772A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.