PA5-95789

antibody from Invitrogen Antibodies

Targeting: POP5

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

PA5-95789

Provider

Invitrogen Antibodies

Product name

POP5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: SIGFAVRYLN AYTGIVLLRC RKEFYQLVWS ALPFITYLEN KGHRYPCFFN TLHVGGTIRT CQKFLIQYNR RQLLILLQNC TDEGEREAIQ KSVTRSCLLE E; Positive Samples: A-549, K562, 293T; Cellular Location: Nucleus, nucleolus

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.62 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POP5 in extracts of various cell lines. Samples were incubated with POP5 Polyclonal antibody (Product # PA5-95789) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.