F54486

antibody from NSJ Bioreagents

Targeting: CDK5 PSSALRE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54486

Provider

NSJ Bioreagents

Product name

CDK5 Antibody

Antibody type

Monoclonal

Description

This highly specific CDK5 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

1552CT262.105.8

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the CDK5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) A549, 2) K562, 3) HeLa and 4) mouse NIH 3T3 cell lysate with CDK5 antibody. Predicted molecular weight ~33 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human A549 cells with CDK5 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skeletal muscle tissue with CDK5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human K562 cells with CDK5 antibody; Blue=isotype control, Green= CDK5 antibody.