PA1-9594

antibody from Invitrogen Antibodies

Targeting: MAPK9 JNK2, p54a, PRKM9, SAPK

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-9594

Provider

Invitrogen Antibodies

Product name

Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-9594 detects Phospho-JNK/SAPK in human, mouse and rat samples.

References

Submitted references

AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma.

Yao CD, Haensel D, Gaddam S, Patel T, Atwood SX, Sarin KY, Whitson RJ, McKellar S, Shankar G, Aasi S, Rieger K, Oro AE
Nature communications 2020 Oct 8;11(1):5079

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Protective effects of Cervus nippon Temminck velvet antler polypeptides against MPP+‑induced cytotoxicity in SH‑SY5Y neuroblastoma cells.

Xin JL, Zhang Y, Li Y, Zhang LZ, Lin Y, Zheng LW
Molecular medicine reports 2017 Oct;16(4):5143-5150

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Western blot analysis of the effects of VAPs on ER stress-related proteins, (A) caspase-12, (B) GRP78, (C) CHOP, (D) p-JNK and (E) beta-actin in SH-SY5Y cells exposed to MPP + . Cells were incubated with 62.5, 125 and 250 ug/ml VAPs together with 120.9 umol/l MPP + . MPP + upregulated the protein expression levels of caspase-12, GRP78 and p-JNK. VAPs downregulated the expression of p-JNK in SH-SY5Y cells in a concentration-dependent manner. Levels of caspase-12 decreased rapidly up to a VAPs concentration of 250 ug/ml. Levels of GRP78 showed no marked reduction following exposure to VAPs. No changes in the expression of CHOP expressions were observed. MPP + , 1-methyl-4-phenylpyridinium; VAPs, velvet antler polypeptides; GRP78, glucose regulated protein 78; CHOP, CCAAT/enhancer binding protein homologous protein; p-JNK, phosphorylated c-Jun N-terminal kinase.