AHO1162

antibody from Invitrogen Antibodies

Targeting: MAPK9 JNK2, p54a, PRKM9, SAPK

ELISA

Antibody data

Submit

Validation data

MAPK9 protein structure - AHO1162 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of JNK2 was achieved by transfecting HeLa with JNK2 specific siRNAs (Silencer® select Product # s11159). Western blot analysis (Fig. a) was performed using whole cell extracts from the JNK2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with JNK2 Monoclonal Antibody (341P16) (Product # AHO1162, 1:1000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to JNK2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-JNK2 Monoclonal Antibody (341P16), (Product # AHO1162) and 50, 44 kDa bands corresponding to JNK2 isoforms were observed in the cell lines tested. Whole cell extracts (30 µg lysate) of HL-60 (Lane 1), Raji (Lane 2), NIH/3T3 (Lane 3), PC-12 (Lane 4), HeLa (Lane 5) and THP-1 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).