Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG51736 - Provider product page
- Provider
- Arigo
- Product name
- anti-SAPK/JNK phospho (Thr183) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells stained with anti-SAPK/JNK (phospho Thr183) antibody ARG51736 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), stained with anti-SAPK/JNK (phospho Thr183) antibody ARG51736.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-SAPK/JNK (phospho Thr183) antibody ARG51736 (left) or the same antibody preincubated with blocking peptide (right).
