LS-C369956

antibody from LSBio

Targeting: MAPK9 JNK2, p54a, PRKM9, SAPK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C369956

Provider

LSBio

Product name

MAPK9 / JNK2 Antibody (aa1-424) LS-C369956

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: MAPK9 antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate Lane 2: mouse brain tissue. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating MAPK9 in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of MAPK9 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: MAPK9 Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating MAPK9 in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of MAPK9 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: MAPK9 Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)