PA1-41234

antibody from Invitrogen Antibodies

Targeting: MAPK9 JNK2, p54a, PRKM9, SAPK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41234

Provider

Invitrogen Antibodies

Product name

Anti-JNK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: A-431 whole cell lysate1.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis for JNK2 using a MAPK9 polyclonal antibody (Product # PA1-41234) at 2 µg/mL on 20 ugs of A431 whole cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-JNK2 Polyclonal Antibody, (Product # PA1-41234) and a 50 kDa band corresponding to JNK2 was observed in the cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), NIH/3T3 (Lane 2), HeLa (Lane 3), THP-1 (Lane 4) in Fig. a and HL-60 (Lane 1), Raji (Lane 2) in Fig. b were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (0.5 µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).