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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-28664 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-JNK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-28664 targets JNK2 in IF and WB applications and shows reactivity with Human and mouse samples. The PA5-28664 immunogen is recombinant fragment corresponding to a region within amino acids 175 and 424 of Human JNK2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.85 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of JNK2/MAPK9 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a JNK2/MAPK9 polyclonal antibody (Product # PA5-28664) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of JNK2/MAPK9 using 30 µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a JNK2/MAPK9 polyclonal antibody (Product # PA5-28664) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of JNK2/MAPK9 in methanol-fixed HeLa cells using a JNK2/MAPK9 polyclonal antibody (Product # PA5-28664) at a 1:200 dilution.
