MAB10548

antibody from Abnova Corporation

Targeting: CDH2 CD325, CDHN, NCAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB10548

Provider

Abnova Corporation

Proper citation

Product name

CDH2 monoclonal antibody, clone 5D5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant CDH2.

Isotype

IgG

Antibody clone number

5D5

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) against A-431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence analysis of A-431 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green). Blue: DRAQ5 fluorescent DNA dye.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) with DAB staining.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human placenta tissues using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Flow cytometric analysis of PC-2 cells using CDH2 monoclonal antibody, clone 5D5 (Cat # MAB10548) (green) and negative control (purple).

Validation comment

Flow Cytometry