sc-8424

CDH2 antibody from Santa Cruz Biotechnology

CD325, CDHN, NCAD

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-8424

Antibody Data

Product number

sc-8424

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-8424, RRID:AB_626778

Product name

Anti-CDH2

Provider product page

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
Stem cells (Dayton, Ohio) 2007 Jun;25(6):1511-20

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 97

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.