GTX19348

antibody from GeneTex

Targeting: CDH2 CD325, CDHN, NCAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX19348

Provider

GeneTex

Proper citation

GeneTex Cat#GTX19348, RRID:AB_423537

Product name

N-Cadherin antibody [8C11]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Chicken/Avian, Hamster, Rabbit

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of N-Cadherin in 25 ug of various lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with N-Cadherin antibody [8C11] at a dilution of 1:1000 overnight at 4¢XC on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a HRP-conjugated secondary antibody for 1 hour. Chemiluminescent detection was performed. Note: analysis indicates both reactive and non-reactive species for N-cadherin.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of HeLa, HUVEC and C2C12 lysates (25 ug per lane) using N-Cadherin antibody [8C11] at a dilution of 1:1000 (HeLa) and 1:500 (HUVEC and C2C12).

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of N-Cadherin in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) N-Cadherin antibody [8C11] at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a proper secondary antibody. F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of N-Cadherin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300ug of lysate with 5ug of N-Cadherin antibody [8C11] overnight on a rocking platform at 4¢XC. The immune complexes were captured on 50ul Protein A/G Agarose , washed extensively, and eluted. The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with N-Cadherin antibody [8C11] at a dilution of 1:1000 overnight rotating at 4¢XC, washed in TBST, and probed with an proper secondary antibody. Chemiluminescent detection was performed.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human breast tissues with an isotype control (A) or N-Cadherin antibody [8C11] (B and C) at a concentration of 2ug/ml. (C) shows the magnified section of (B). To expose target proteins, antigen retrieval was performed using HEIR with a buffer (pH 6.2).