350816

antibody from BioLegend

Targeting: CDH2 CD325, CDHN, NCAD

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

350816

Provider

BioLegend

Proper citation

BioLegend Cat#350816, RRID:AB_2563226

Product name

Alexa Fluor® 594 anti-human CD325 (N-Cadherin)

Antibody type

Monoclonal

Antigen

Recombinant human N-cadherin extracellular domain

Description

CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

8C11

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human acute myeloid leukemia cell line (KG1) cells were fixed with 2% paraformaldehyde (PFA) and blocked for 30 minutes with 5% FBS. Then the cells were stained with 5 µg/ml of Alexa Fluor® 594 anti-human CD325 (N-Cadherin) (clone 8C11) (red) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.

Conjugate

Yellow dye