Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12-3259-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD325 (N-Cadherin) Monoclonal Antibody (8C11), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin. CD325 is a 130 kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells. Applications Reported: This 8C11 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 8C11 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 8C11
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ
The Journal of biological chemistry 2003 May 9;278(19):17269-76
The Journal of biological chemistry 2003 May 9;278(19):17269-76
N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.
Puch S, Armeanu S, Kibler C, Johnson KR, Müller CA, Wheelock MJ, Klein G
Journal of cell science 2001 Apr;114(Pt 8):1567-77
Journal of cell science 2001 Apr;114(Pt 8):1567-77
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of HeLa cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD325 (N-Cadherin) PE (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Yellow dye