Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-3259-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD325 (N-Cadherin) Monoclonal Antibody (8C11), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin. CD325 is a 130 kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells. Applications Reported: This 8C11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 8C11 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 8C11 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8C11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ
The Journal of biological chemistry 2003 May 9;278(19):17269-76
The Journal of biological chemistry 2003 May 9;278(19):17269-76
N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.
Puch S, Armeanu S, Kibler C, Johnson KR, Müller CA, Wheelock MJ, Klein G
Journal of cell science 2001 Apr;114(Pt 8):1567-77
Journal of cell science 2001 Apr;114(Pt 8):1567-77
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of HeLa cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD325 (N-Cadherin) Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.