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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503775 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503775, RRID:AB_11125904
- Product name
- CDH2 (N Cadherin) mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)
- Antibody type
- Monoclonal
- Description
- CDH2 (N Cadherin) mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CDH2
- Isotype
- IgG
- Antibody clone number
- OTI3B10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Chemotherapy enhances tumor vascularization via Notch signaling-mediated formation of tumor-derived endothelium in breast cancer.
Zhang P, He D, Chen Z, Pan Q, Du F, Zang X, Wang Y, Tang C, Li H, Lu H, Yao X, Jin J, Ma X
Biochemical pharmacology 2016 Oct 15;118:18-30
Biochemical pharmacology 2016 Oct 15;118:18-30
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH2 (RC207170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDH2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503775)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503775)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC207170 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CDH2 antibody(TA503775), and then analyzed by flow cytometry.
- Validation comment
- FC
