- PA5-19486 - Invitrogen Antibodies CDH2 antibody

Submitted references Neonatal Hyperoxia Downregulates Claudin-4, Occludin, and ZO-1 Expression in Rat Kidney Accompanied by Impaired Proximal Tubular Development.

Xu X, Zhang X, Gao L, Liu C, You K
Oxidative medicine and cellular longevity 2020;2020:2641461

H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.

Bacci L, Aiello A, Ripoli C, Loria R, Pugliese D, Pierconti F, Rotili D, Strigari L, Pinto F, Bassi PF, Mai A, Grassi C, Pontecorvi A, Falcioni R, Farsetti A, Nanni S
International journal of molecular sciences 2019 Aug 17;20(16)

VCAM-1 induces signals that stimulate ZO-1 serine phosphorylation and reduces ZO-1 localization at lung endothelial cell junctions.

Abdala-Valencia H, Kountz TS, Marchese ME, Cook-Mills JM
Journal of leukocyte biology 2018 Jul;104(1):215-228

ZEB1 Regulates Multiple Oncogenic Components Involved in Uveal Melanoma Progression.

Chen Y, Lu X, Montoya-Durango DE, Liu YH, Dean KC, Darling DS, Kaplan HJ, Dean DC, Gao L, Liu Y
Scientific reports 2017 Mar 3;7(1):45

Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.

Fang S, Yu L, Mei H, Yang J, Gao T, Cheng A, Guo W, Xia K, Liu G
Oncology letters 2016 Dec;12(6):5007-5014

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of N-cadherin was performed by loading human fetal astrocyte cell lysate (Lane 1) and 2.5 µg of human brain tissue extract (Lane 2) per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked for 1 hour at room temperature. The membrane was probed with an N-cadherin polyclonal antibody (Product # PA5-19486) at a concentration for 1 µg/mL overnight at 4°C, washed in PBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature Detection was performed using ECL substrate (Product # 32106). Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of N-cadherin was performed by loading human fetal astrocyte cell lysate (Lane 1) and 2.5 µg of human brain tissue extract (Lane 2) per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked for 1 hour at room temperature. The membrane was probed with an N-cadherin polyclonal antibody (Product # PA5-19486) at a concentration for 1 µg/mL overnight at 4°C, washed in PBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature Detection was performed using ECL substrate (Product # 32106). Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extract (30 µg lysate) of Mouse Brain (Lane 1). The blot was probed with Anti-N-cadherin Polyclonal Antibody (Product # PA5-19486, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 130 kDa band corresponding to N-cadherin was observed in the tissue tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Mouse Brain Tissue Lysate using Product # PA5-19486, N Cadherin primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Rat Brain Tissue Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of N-cadherin was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with N-cadherin Monoclonal Antibody (Product # PA5-19486) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Liver Cancer tissue using Product # PA5-19486, anti-N Cadherin antibody. Primary antibody was used at a concentration of 1 µg/mL and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. Cisplatin promotes EMT-TFs in osteosarcoma. (A) The relative expression of EMT-TFs, including Snail/Slug and Zeb1/2 were observed to be significantly upregulated in the cisplatin treated cells by quantitative polymerase chain reaction. **P

PA5-19486

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