Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1388 - Provider product page
- Provider
- R&D Systems
- Product name
- Human N-Cadherin Propeptide Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human N-Cadherin Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-8, rhCadherin-11, and rhR-Cadherin is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P19022
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cadherin adhesion depends on a salt bridge at the N-terminus.
Harrison OJ, Corps EM, Kilshaw PJ
Journal of cell science 2005 Sep 15;118(Pt 18):4123-30
Journal of cell science 2005 Sep 15;118(Pt 18):4123-30
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Pro-N-Cadherin by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human N-Cadherin Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1388) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Pro-N-Cadherin was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- N-Cadherin in Human Brain. N-Cadherin was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 15 µg/mL Sheep Anti-Human N-Cadherin Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1388) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to endothelial cells in a capillary. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.