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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [2]
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- Product number
- FAB6426A - Provider product page
- Provider
- R&D Systems
- Product name
- Human N-Cadherin APC-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat N-Cadherin in Western blots. In direct ELISA, less than 1% cross-reactivity with recombinant human (rh) E-Cadherin, rhR-Cadherin, and rhCadherin-13 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Red dye
- Antigen sequence
P19022- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references The p63 protein isoform ΔNp63α inhibits epithelial-mesenchymal transition in human bladder cancer cells: role of MIR-205.
Tran MN, Choi W, Wszolek MF, Navai N, Lee IL, Nitti G, Wen S, Flores ER, Siefker-Radtke A, Czerniak B, Dinney C, Barton M, McConkey DJ
The Journal of biological chemistry 2013 Feb 1;288(5):3275-88
The Journal of biological chemistry 2013 Feb 1;288(5):3275-88
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of N-Cadherin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human N-Cadherin APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6426A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- N-Cadherin Specificity is Shown by Flow Cytometry in Knockout Cell Line N-Cadherin knockout HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB6426A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). No staining in the N-Cadherin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
