H00003113-M03

antibody from Abnova Corporation

Targeting: HLA-DPA1 HLA-DP1A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003113-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003113-M03, RRID:AB_1136946

Product name

HLA-DPA1 monoclonal antibody (M03), clone 1E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HLA-DPA1.

Antigen sequence

MRPEDRMFHIRAVILRALSLAFLLSLRGAGAIKAD
HVSTYAAFVQTHRPTGEFMFEFDEDEQFYVDLDKK
ETVWHLEEFGRAFSFEAQGGLANIAILNNNLNTLI
QRSNHTQAANDPPEVTVFPKEPVELGQPNTLICHI
DRFFPPVLNVTWLCNGEPVTEGVAESLFLPRTDYS
FHKFHYLTFVPSAEDVYDCRVEHWGLDQPLLKHWE
AQEPIQMPETTETVLCALGLVLGLVGIIVGTVLII
KSLRSGHDPRAQGPL


Isotype

IgG

Antibody clone number

1E3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol