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- Validations
- Western blot [3]
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- Product number
- MA1-40198 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transferrin Receptor Monoclonal Antibody (3B82A1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B82A1
- Vial size
- 100 µg
- Concentration
- 0.1 mg/mL
- Storage
- 4° C
Submitted references Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells.
Mehta K, Greenwell P, Renshaw D, Busbridge M, Garcia M, Farnaud S, Patel VB
Blood cells, molecules & diseases 2015 Aug;55(2):110-8
Blood cells, molecules & diseases 2015 Aug;55(2):110-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of Transferrin Receptor was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR944258_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Transferrin Receptor was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa Transferrin Receptor KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Transferrin Receptor Monoclonal Antibody (3B82A1) (Product # MA1-40198, 1:50 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to Transferrin Receptor.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Transferrin Receptor was achieved by transfecting HeLa with Transferrin Receptor specific siRNAs (Silencer® select Product # S725, S726). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Transferrin Receptor knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Transferrin Receptor Monoclonal Antibody (3B82A1) (Product # MA1-40198, 1:50 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Transferrin Receptor.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Transferrin Receptor Monoclonal Antibody (3B82A1)(Product # MA1-40198) and a 84kDa band corresponding to Transferrin Receptor was observed across cell line tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), U-87 MG (Lane 3) and MCF-7 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody ( 1:50 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
