Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB200-585AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TfR (Transferrin R) Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Labels dividing cells of various types but also binds to a number of non-dividing normal tissues and endothelium of brain capillaries.
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [Alexa Fluor® 647] [NB200-585AF647] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.