R31646

antibody from NSJ Bioreagents

Targeting: TFRC CD71, p90, TFR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31646

Provider

NSJ Bioreagents

Product name

Transferrin Receptor Antibody

Antibody type

Polyclonal

Description

This highly specific Transferrin Receptor antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Transferrin Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Transferrin Receptor antibody and Lane 1: HeLa; 2: Jurkat; 3: Raji; 4: HL-60; 5: K562; 6: HEPG2; 7: human placenta; 8: CEM lysate. Predicted molecular weight 85~100 kDa

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Transferrin Receptor antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human K562, 3) human HT1080, 4) rat thymus, 5) mouse RAW264.7 and 6) mouse SP2/0 cell lysate with Transferrin Receptor antibody. Predicted molecular weight 85~95 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A431 cells with Transferrin Receptor antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human placental tissue with Transferrin Receptor antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Transferrin Receptor antibody testing of human placenta tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Transferrin Receptor antibody testing of human placenta tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of frozen human placenta with Transferrin Receptor antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human SiHa cells with Transferrin Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Transferrin Receptor antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-87 MG cells with Transferrin Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Transferrin Receptor antibody.