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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V2260 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Transferrin Receptor Antibody / CD71
- Antibody type
- Monoclonal
- Description
- This highly specific Transferrin Receptor antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- DF1513
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the Transferrin receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Jurkat, 2) Raji and 3) ThP-1 cell lysate with Transferrin receptor antibody (clone DF1513). Predicted molecular weight: 85-95 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human Jurkat cells with Transferrin receptor antibody (clone DF1513, green) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry staining of human Jurkat cells with Transferrin receptor antibody; Red=isotype control, Blue= cells alone and Orange= Transferrin receptor antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Transferrin receptor antibody (clone DF1513) as confirmation of integrity and purity.
