MA1-19299

antibody from Invitrogen Antibodies

Targeting: TFRC CD71, p90, TFR1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19299

Provider

Invitrogen Antibodies

Product name

Transferrin Receptor Monoclonal Antibody (MEM-189)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with an extracellular epitope on CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor. Western blot should be performed under non-reducing conditions.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MEM-189

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Transferrin Receptor was performed using 70% confluent log phase HeLa cells treated with Brefeldin A (5 µg/mL for 30 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Transferrin Receptor Monoclonal Antibody (MEM-189) (Product # MA1-19299) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased Transferrin Receptor expression and localization to golgi and cytoplasm upon treatment with Brefeldin A. Panel e shows untreated cells with lower expression of Transferrin Receptor. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of K562 cells stained using anti-human CD71 (MEM-189) purified Monoclonal antibody (Product # MA1-19299) (concentration in sample 4 µg/mL, GAM APC, red) from K562 cells unstained by primary antibody (GAM APC, grey) in flow cytometry analysis (surface staining).