- PA5-27739 - Invitrogen Antibodies TFRC antibody

Lara Ordóñez AJ, Fasiczka R, Fernández B, Naaldijk Y, Fdez E, Blanca Ramírez M, Phan S, Boassa D, Hilfiker S
Biology open 2022 Aug 15;11(8)

Balgoma D, Kullenberg F, Calitz C, Kopsida M, Heindryckx F, Lennernäs H, Hedeland M
Cells 2021 May 11;10(5)

Wang YQ, Chang SY, Wu Q, Gou YJ, Jia L, Cui YM, Yu P, Shi ZH, Wu WS, Gao G, Chang YZ
Frontiers in aging neuroscience 2016;8:308

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Transferrin Receptor/CD71 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Transferrin Receptor/CD71 polyclonal antibody (Product # PA5-27739) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Transferrin-Receptor was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Transferrin Receptor Polyclonal Antibody (Product # PA5-27739) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Transferrin Receptor Polyclonal Antibody (Product # PA5-27739). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin Receptor Polyclonal Antibody (Product # PA5-27739) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Transferrin Receptor Polyclonal Antibody (Product # PA5-27739). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin Receptor Polyclonal Antibody (Product # PA5-27739) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Transferrin Receptor Polyclonal Antibody detects CD71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD71 protein stained by Transferrin Receptor Polyclonal Antibody (Product # PA5-27739) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Effects of FtMt on the LIP level and iron metabolism under erastin treatment. (A) LIP levels were determined by the quenching of calcein-AM fluorescence method using fluorescence spectrophotometer. The LIP level was presented as mean +- SD; n = 3 ( ** p < 0.01 vs. the untreated cells of same genotype; ## p < 0.01 vs. the erastin-treated vector controls). Ferritin (B) and TfR1 (C) levels were determined by western blots. A representative blot image for each protein and its respective beta-actin was shown. The expression levels in different groups were calculated by normalizing the specific bands to their respective beta-actin bands, and presented as means +- SD, n = 6 ( ** p < 0.01 vs. the untreated cells of same genotype; ## p < 0.01 vs. the erastin-treated vector controls).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 9 Doxorubicin (DOX) treatment increases the expression of transferrin receptor. ( A ) Percentage of transferrin receptor-positive staining in the liver of the mouse model untreated (white), induced with hepatocellular carcinoma (HCC, light red), and treated with DOX after induction of HCC (dark red). ( B ) Representative images of the treatments at large scale (upper row) and detailed scale (lower row). Five replicates were performed; the bar represents the average, and the error bars represent the standard error of the mean.

PA5-27739