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LearnPA5-57729
antibody from Invitrogen Antibodies
Targeting: SHLD2
bA163M19.1, FAM35A, FAM35A1, MGC5560, RINN2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-57729 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM35A/FAM35B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FLANCMNRHV HVKDDFVRSV SETQNIESQK IHSSRLSDIT SSNMQICGFK STVPHFTEEE KYQKLLSENK IRDEQPKHQP D Highest antigen sequence identity to the following orthologs: Mouse - 45%, Rat - 50%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAM35A/FAM35B in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a FAM35A/FAM35B Polyclonal Antibody (Product # PA5-57729).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FAM35A/FAM35B in human cell line U-2 OS shows positivity in actin filaments & nucleus but excluded from the nucleoli. Samples were probed using a FAM35A/FAM35B Polyclonal Antibody (Product # PA5-57729).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FAM35A/FAM35B in human urinary bladder using FAM35A/FAM35B Polyclonal Antibody (Product # PA5-57729) shows strong cytoplasmic positivity in urothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FAM35A/FAM35B in human kidney using FAM35A/FAM35B Polyclonal Antibody (Product # PA5-57729) shows moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FAM35A/FAM35B in human cerebellum using FAM35A/FAM35B Polyclonal Antibody (Product # PA5-57729) shows moderate ytoplasmic positivity in Purkinje cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FAM35A/FAM35B in human skeletal muscle using FAM35A/FAM35B Polyclonal Antibody (Product # PA5-57729) shows no positivity in myocytes as expected.
