H00002664-M08

antibody from Abnova Corporation

Targeting: GDI1 FLJ41411, GDIL, MRX41, MRX48, OPHN2, RABGDIA, XAP-4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002664-M08

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002664-M08, RRID:AB_518829

Product name

GDI1 monoclonal antibody (M08), clone 1H3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GDI1.

Antigen sequence

MDEEYDVIVLGTGLTECIPSGIMSVNGKKVLHMDR
NPYYGGESSSITPLEELYKRFQLLEGPPESMGRGR
DWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVV
EGSFVYKGGKIYKVPSTETEALASNLMGMFEKRRF
RKFLVFVANFDENDPKTFEGVDPQTTSMRDVYRKF
DLGQDVIDFTGHALALYRTDDYLDQPCLETVNRIK
LYSESLARYGKSPYLYPLYGLGELPQGFARLSAIY
GGTYMLNKPVDDIIMENGKVVGVKSEGEVARCKQL
ICDPSYIPDRVRKAGQVIRIICILSHPIKNTNDAN
SCQIIIPQNQVNRKSDIYVCMISYAHNVAAQGKYI
AIASTTVETTDPEKEVEPALELLGPIDQKFVAISD
LYEPIDDGCESQVFCSCSYDATTHFETTCNDIKDI
YKRMAGTAFDFENMKRKQNDVFGEAEQ


Isotype

IgG

Antibody clone number

1H3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GDI1 monoclonal antibody (M08), clone 1H3 Western Blot analysis of GDI1 expression in IMR-32 ( Cat # L008V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GDI1 expression in transfected 293T cell line by GDI1 monoclonal antibody (M08), clone 1H3.Lane 1: GDI1 transfected lysate(50.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GDI1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol