PA5-81504

antibody from Invitrogen Antibodies

Targeting: GDI1 FLJ41411, GDIL, MRX41, MRX48, OPHN2, RABGDIA, XAP-4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81504

Provider

Invitrogen Antibodies

Product name

GDI1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GDI1 in Lane A: NIHT3 Whole Cell lysate (30 µg). Samples were probed using a GDI1 Polyclonal Antibody (Product # PA5-81504) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:51 kDa. Observed band size:47 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GDI1 Polyclonal Antibody (Product # PA5-81504) at 1:500 dilution. Lane A: NIHT3 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 47 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of GDI1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with GDI1 Polyclonal Antibody (Product # PA5-81504, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human GDI1 in human gastric cancer with GDI1 Polyclonal Antibody (Product # PA5-81504, 1:2,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GDI1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg U87MG Whole Cell Lysate 2 µL with GDI1 Polyclonal Antibody (Product # PA5-81504) and 15 µL of 50 % Protein G agarose. Primary antibody: GDI1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GDI1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg U87MG Whole Cell Lysate 2 µL with GDI1 Polyclonal Antibody (Product # PA5-81504) and 15 µL of 50 % Protein G agarose. Primary antibody: GDI1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa.