10990-2-AP

antibody from Proteintech Group

Targeting: SKP1 EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10990-2-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10990-2-AP, RRID:AB_2187492

Product name

SKP1 antibody

Antibody type

Polyclonal

Description

SKP1 antibody (Cat. #10990-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Transforming growth factor-β3 attenuates septic cardiomyopathy by reversing cardiomyocyte metabolic reprogramming through Smad7 signaling.

Zhang H, Xu J, Xu B, Shang X
Molecular and cellular biochemistry 2026 Mar;481(3):1241-1250

---

IFNα/JAK/STAT1 Axis-Induced FBXO4 Modulates Muscle Cell Differentiation via β-Catenin Degradation in Dermatomyositis.

Yin L, Yang H, Fu M, Bai Y, Hu N, Sun H
Journal of inflammation research 2025;18:8527-8539

---

Isoorientin Ameliorates Macrophage Pyroptosis and Atherogenesis by Reducing KDM4A Levels and Promoting SKP1-Cullin1-F-box E3 Ligase-mediated NLRP3 Ubiquitination.

Wang X, Xie N, Zhang H, Zhou W, Lei J
Inflammation 2025 Oct;48(5):3629-3648

---

S-phase kinase-associated protein 1 inhibits orbital fibroblasts adipogenesis to improve thyroid-associated ophthalmopathy (TAO).

Lu S, Xiong W, Yi J, Liu S, Zhang F
Biochimica et biophysica acta. Molecular cell research 2025 Jun;1872(5):119937

---

Subversion of the host endocytic pathway by Legionella pneumophila-mediated ubiquitination of Rab5.

Tanaka S, Oide H, Ikeda S, Tagaya M, Nagai H, Kubori T, Arasaki K
The Journal of cell biology 2025 Apr 7;224(4)

---

A Circular RNA Generated from Nebulin (NEB) Gene Splicing Promotes Skeletal Muscle Myogenesis in Cattle as Detected by a Multi-Omics Approach.

Huang K, Li Z, Zhong D, Yang Y, Yan X, Feng T, Wang X, Zhang L, Shen X, Chen M, Luo X, Cui K, Huang J, Rehman SU, Jiang Y, Shi D, Pauciullo A, Tang X, Liu Q, Li H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(3):e2300702

---

Recruitment of FBXO22 for targeted degradation of NSD2.

Nie DY, Tabor JR, Li J, Kutera M, St-Germain J, Hanley RP, Wolf E, Paulakonis E, Kenney TMG, Duan S, Shrestha S, Owens DDG, Maitland MER, Pon A, Szewczyk M, Lamberto AJ, Menes M, Li F, Penn LZ, Barsyte-Lovejoy D, Brown NG, Barsotti AM, Stamford AW, Collins JL, Wilson DJ, Raught B, Licht JD, James LI, Arrowsmith CH
Nature chemical biology 2024 Dec;20(12):1597-1607

---

Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer.

Huang D, Wu PE, Chen ZJ, Pang YC, Xu ZW, Tan J, Jiang ZH, Yang BB, Zhan R, Xu H, Liu YQ
Molecular nutrition & food research 2023 Sep;67(18):e2300061

---

HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression.

He L, Li H, Li C, Liu ZK, Lu M, Zhang RY, Wu D, Wei D, Shao J, Liu M, Wei HL, Zhang C, Wang Z, Kong LM, Chen ZN, Bian H
Cancer communications (London, England) 2023 Sep;43(9):981-1002

---

Recruitment of FBXO22 for Targeted Degradation of NSD2.

Nie DY, Tabor JR, Li J, Kutera M, St-Germain J, Hanley RP, Wolf E, Paulakonis E, Kenney TMG, Duan S, Shrestha S, Owens DDG, Pon A, Szewczyk M, Lamberto AJ, Menes M, Li F, Barsyte-Lovejoy D, Brown NG, Barsotti AM, Stamford AW, Collins JL, Wilson DJ, Raught B, Licht JD, James LI, Arrowsmith CH
bioRxiv : the preprint server for biology 2023 Nov 30;

---

Brusatol has therapeutic efficacy in non-small cell lung cancer by targeting Skp1 to inhibit cancer growth and metastasis.

Xing S, Nong F, Wang Y, Huang D, Qin J, Chen YF, He DH, Wu PE, Huang H, Zhan R, Xu H, Liu YQ
Pharmacological research 2022 Feb;176:106059

---

Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.

Lin XT, Yu HQ, Fang L, Tan Y, Liu ZY, Wu D, Zhang J, Xiong HJ, Xie CM
eLife 2021 Nov 15;10

---

Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.

Zhu H, Zhang H, Pei Y, Liao Z, Liu F, Su C, Liu Y, Dong R, Song J, Zhang X, Fan Y, Liang H, Zhang B, Chen X
Journal of experimental & clinical cancer research : CR 2021 Feb 4;40(1):57

---

The ubiquitin ligase SCF(FBXW7α) promotes GATA3 degradation.

Song N, Cao C, Tang Y, Bi L, Jiang Y, Zhou Y, Song X, Liu L, Ge W
Journal of cellular physiology 2018 Mar;233(3):2366-2377

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 10990-2-AP(SKP1 antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SKP1 antibody 10990-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 10990-2-AP(SKP1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue