32-3800

antibody from Invitrogen Antibodies

Targeting: SKP1 EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

32-3800

Provider

Invitrogen Antibodies

Product name

SKP1 Monoclonal Antibody (1C10F4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

SKP1 / p19A Antibody (1C10F4), Prod# 32-3800 specifically detects SKP1 in human cells. The antibody may also result in weak detection of 2 unknown bands at ~37kD.

Antibody clone number

1C10F4

Concentration

0.5 mg/mL

References

Submitted references

F-box protein FBXL16 binds PP2A-B55α and regulates differentiation of embryonic stem cells along the FLK1+ lineage.

Honarpour N, Rose CM, Brumbaugh J, Anderson J, Graham RL, Sweredoski MJ, Hess S, Coon JJ, Deshaies RJ
Molecular & cellular proteomics : MCP 2014 Mar;13(3):780-91

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Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy.

Chen Q, Xie W, Kuhn DJ, Voorhees PM, Lopez-Girona A, Mendy D, Corral LG, Krenitsky VP, Xu W, Moutouh-de Parseval L, Webb DR, Mercurio F, Nakayama KI, Nakayama K, Orlowski RZ
Blood 2008 May 1;111(9):4690-9

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Hierarchical order of phosphorylation events commits Cdc25A to betaTrCP-dependent degradation.

Donzelli M, Busino L, Chiesa M, Ganoth D, Hershko A, Draetta GF
Cell cycle (Georgetown, Tex.) 2004 Apr;3(4):469-71

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Degradation of Cdc25A by beta-TrCP during S phase and in response to DNA damage.

Busino L, Donzelli M, Chiesa M, Guardavaccaro D, Ganoth D, Dorrello NV, Hershko A, Pagano M, Draetta GF
Nature 2003 Nov 6;426(6962):87-91

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SKP-1/p19A and Cullin-1 was performed by loading 10 µg of whole cell lysates from DMSO, MLN4924 or MG132 treated U2OS cells onto a SDS PAGE gel. Proteins were transferred, blocked and endogenous SKP-1 (right panel) and Cullin-1 (left panel) were detected using a SKP-1 mouse monoclonal antibody (Product # 32-3800) or CUL-1 mouse monoclonal antibody (Product # 32-2400) at dilutions of 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2. FBXL16 is a cytoplasmic protein that binds SKP1 and PP2A but does not bind CUL1. A , expression of myc-FBXL16. Total cell lysate was prepared from 3T3 cells that were either mock-transfected or stably transfected with plasmids that contained the coding sequences for either myc-SKP2 or myc-FBXL16. Lysates were evaluated via SDS-PAGE followed by immunoblot with anti-myc. B , myc-FBXL16 is a cytosolic protein. Cytosolic (Cyt) and nuclear (Nuc) fractions prepared from 3T3 cells stably expressing myc-FBXL16 were fractionated by means of SDS-PAGE and immunoblotted for myc-FBXL16, alpha-actin (a cytosolic marker), and SP1 (a nuclear marker). C , myc-FBXL16 binds SKP1 and PP2A but not CUL1. Total cell lysates from 293 cells that were either mock-transfected or transfected with plasmids that contained the coding sequences for either myc-SKP2 or myc-FBXL16 were subjected to immunoprecipitation with anti-myc. Immunoprecipitates were fractionated via SDS-PAGE and immunoblotted with antibodies against SKP1, CUL1, and PP2A subunit A. D , FBXL16, but not SKP2, co-immunoprecipitates serine/threonine phosphatase activity. Total cell lysates from 293 cells that were either mock-transfected or transfected with plasmids that contained the coding sequences for myc-SKP2, myc-FBXL16, or HA-B55alpha (included as a positive control) were subjected to immunoprecipitation with either anti-myc (SKP2 and FBXL16) or anti-HA (B55alpha). Immunoprecipitates were assayed for their content of serine/th