TA322455

antibody from OriGene

Targeting: SKP1 EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA322455

Provider

OriGene

Product name

Anti-SKP1 rabbit polyclonal antibody

Antibody type

Polyclonal

Description

Anti-SKP1 rabbit polyclonal antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

SKP1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1.1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 19kDa. Positive control: Mouse brain tissue and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SKP1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)

Validation comment

IHC