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LearnMAB10319
antibody from Abnova Corporation
Targeting: SKP1
EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10319 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10319, RRID:AB_11188093
- Product name
- SKP1 monoclonal antibody, clone 4E11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant SKP1.
- Isotype
- IgG
- Antibody clone number
- 4E11
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) against HeLa (1), NIH/3T3 (2), A-431 (3), RAJI (4), PC-12 (5), COS-7 (6), MCF-7 (7) and HepG2 (8) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) against recombinant human SKP1 protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green) and negative control (red).
- Validation comment
- Flow Cytometry
