MA5-15928

antibody from Invitrogen Antibodies

Targeting: SKP1 EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15928

Provider

Invitrogen Antibodies

Product name

SKP1 Monoclonal Antibody (4E11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15928 targets SKP1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15928 immunogen is purified recombinant fragment of human SKP1 expressed in E. Coli. . MA5-15928 detects SKP1 which has a predicted molecular weight of approximately 19kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

4E11

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression.

Pegoraro A, Orioli E, De Marchi E, Salvestrini V, Milani A, Di Virgilio F, Curti A, Adinolfi E
Cell death & disease 2020 Oct 18;11(10):876

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Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.

Liu X, Reitsma JM, Mamrosh JL, Zhang Y, Straube R, Deshaies RJ
Molecular cell 2018 Mar 1;69(5):773-786.e6

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SKP1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SKP1 Mouse Monoclonal Antibody (4E11) (Product # MA5-15928) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using SKP1 monoclonal antibody (Product # MA5-15928) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of NIH/3T3 cells using SKP1 monoclonal antibody (Product # MA5-15928) (green) and negative control (red).