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LearnPA5-28489
antibody from Invitrogen Antibodies
Targeting: SKP1
EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-28489 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SKP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, MCF-7, NIH-3T3. Predicted reactivity: Rat (100%), Zebrafish (99%), Xenopus laevis (100%), Dog (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SKP1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a SKP1 Polyclonal Antibody (Product # PA5-28489) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SKP1 Polyclonal Antibody (Product # PA5-28489). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 12% SDS PAGE. SKP1 Polyclonal Antibody (Product # PA5-28489) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SKP1 Polyclonal Antibody (Product # PA5-28489). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. Lane D: MCF-7. 12% SDS PAGE. SKP1 Polyclonal Antibody (Product # PA5-28489) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SKP1 Polyclonal Antibody detects SKP1 protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. SKP1 Polyclonal Antibody (Product # PA5-28489) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
