H00000212-M01

antibody from Novus Biologicals

Targeting: ALAS2 ASB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000212-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00000212-M01, RRID:AB_536712

Product name

Mouse Monoclonal ALAS2 Antibody

Antibody type

Monoclonal

Description

IgG purified. ALAS2 - aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Emodin can induce K562 cells to erythroid differentiation and improve the expression of globin genes.

Ma YN, Chen MT, Wu ZK, Zhao HL, Yu HC, Yu J, Zhang JW
Molecular and cellular biochemistry 2013 Oct;382(1-2):127-36

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Hypoxic induction of human erythroid-specific δ-aminolevulinate synthase mediated by hypoxia-inducible factor 1.

Zhang FL, Shen GM, Liu XL, Wang F, Zhao HL, Yu J, Zhang JW
Biochemistry 2011 Feb 22;50(7):1194-202

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ALAS2 Antibody (6C1) [H00000212-M01] - Analysis of ALAS2 expression in transfected 293T cell line by ALAS2 monoclonal antibody (M01), clone 6C1.Lane 1: ALAS2 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ALAS2 Antibody (6C1) [H00000212-M01] - ALAS2 monoclonal antibody (M01), clone 6C1. Analysis of ALAS2 expression in Raw 264.7.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: ALAS2 Antibody (6C1) [H00000212-M01] - Detection limit for recombinant GST tagged ALAS2 is approximately 0.1ng/ml as a capture antibody.