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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-96460 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPS10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEIV PATLRRSRPE TGRPRPKGLE GERPARLTRG EADRDTYRRS AVPPGADKKA EAGAGSATEF QFRGGFGRGR GQPPQ; Positive Samples: MCF7, HL-60, A-549, HT-29, NCI-H460, Mouse spleen, Mouse heart, Mouse liver, Rat liver; Cellular Location: Cytoplasm, Nucleus, nucleolus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 5.89 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RPS10 was performed in MCF7 cells using RPS10 Polyclonal Antibody (Product # PA5-96460).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPS10 in paraffin-embedded mouse brain. Samples were incubated with RPS10 Polyclonal antibody (Product # PA5-96460) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPS10 in paraffin-embedded rat brain. Samples were incubated with RPS10 Polyclonal antibody (Product # PA5-96460) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RPS10 was performed in 200 µg extracts of MCF-7 cells using RPS10 Polyclonal Antibody (Product # PA5-96460). Western blot was performed from the immunoprecipitate using RPS10 Polyclonal Antibody at a dilution of 1:1000.
