PA5-96460

antibody from Invitrogen Antibodies

Targeting: RPS10 MGC88819, S10

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96460

Provider

Invitrogen Antibodies

Product name

RPS10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEIV PATLRRSRPE TGRPRPKGLE GERPARLTRG EADRDTYRRS AVPPGADKKA EAGAGSATEF QFRGGFGRGR GQPPQ; Positive Samples: MCF7, HL-60, A-549, HT-29, NCI-H460, Mouse spleen, Mouse heart, Mouse liver, Rat liver; Cellular Location: Cytoplasm, Nucleus, nucleolus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

5.89 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPS10 in various lysates. Samples were incubated with RPS10 Polyclonal antibody (Product # PA5-96460) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPS10 was performed in MCF7 cells using RPS10 Polyclonal Antibody (Product # PA5-96460).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPS10 in HeLa cells. Samples were incubated with RPS10 Polyclonal antibody (Product # PA5-96460) using a dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RPS10 in 200 μg extracts of MCF-7 cells. Samples were precipitated with 1 μg RPS10 Polyclonal antibody (Product # PA5-96460). Western blot was performed from the immunoprecipitate using RPS10 Polyclonal antibody (Product # PA5-96460) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS10 in paraffin-embedded mouse brain. Samples were incubated with RPS10 Polyclonal antibody (Product # PA5-96460) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS10 in paraffin-embedded rat brain. Samples were incubated with RPS10 Polyclonal antibody (Product # PA5-96460) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS10 in paraffin-embedded mouse brain. Samples were incubated with RPS10 Polyclonal antibody (Product # PA5-96460) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of RPS10 was performed in 200 µg extracts of MCF-7 cells using RPS10 Polyclonal Antibody (Product # PA5-96460). Western blot was performed from the immunoprecipitate using RPS10 Polyclonal Antibody at a dilution of 1:1000.