PA5-100204

antibody from Invitrogen Antibodies

Targeting: TMEM158 p40BBp, RIS1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-100204

Provider

Invitrogen Antibodies

Product name

TMEM158 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total TM158.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TMEM158 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TMEM158 polyclonal antibody (Product # PA5-100204) using a dilution of 1:100 (primary, 1 hr, 37°C), 1:200 (tubulin), and followed by goat anti-mouse Alexa Fluor 594 and goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TMEM158 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TMEM158 polyclonal antibody (Product # PA5-100204) using a dilution of 1:100 (primary, 1 hr, 37°C), 1:200 (tubulin), and followed by goat anti-mouse Alexa Fluor 594 and goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TMEM158 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TMEM158 polyclonal antibody (Product # PA5-100204) using a dilution of 1:100 (primary, 1 hr, 37°C), 1:200 (tubulin), and followed by goat anti-mouse Alexa Fluor 594 and goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugated.