Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47771 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BTF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BTF3 in HeLa human cervical epithelial carcinoma cell line, SK‚BR‚3 human breast cancer cell line, BaF3 mouse pro‚B cell line, and Y3‚Ag rat myeloid cell line. Samples were incubated in BTF3 polyclonal antibody (Product # PA5-47771) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for BTF3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BTF3 in HeLa human cervical epithelial carcinoma cell line, SK‚BR‚3 human breast cancer cell line, BaF3 mouse pro‚B cell line, and Y3‚Ag rat myeloid cell line. Samples were incubated in BTF3 polyclonal antibody (Product # PA5-47771) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for BTF3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BTF3 in immersion fixed paraffin-embedded sections of human pancreas cancer tissue. Samples were incubated in BTF3 polyclonal antibody (Product # PA5-47771) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-basic. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BTF3 in immersion fixed paraffin-embedded sections of human pancreas cancer tissue. Samples were incubated in BTF3 polyclonal antibody (Product # PA5-47771) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-basic. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).