A302-319A

antibody from Invitrogen Antibodies

Targeting: BTF3 BTF3a, BTF3b, NACB

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-319A

Provider

Invitrogen Antibodies

Product name

BTF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BTF3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- BTF3 (Cat. No. A302-319A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BTF3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody A302-319A used for IP at 10 µg/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody A302-318A, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, A302-318A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.