Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-28700 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28700, RRID:AB_1913922
- Product name
- Rabbit Polyclonal BTF3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BTF3 Antibody [NBP1-28700] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody NBP1-28700 used for IP at 10 ug/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody NBP1-28716, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, NBP1-28716 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BTF3 Antibody [NBP1-28700] - Sample: FFPE section of human breast carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- BTF3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB