Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- AF1120 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Frizzled-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) Frizzled-2, rmFrizzled-4, rmFrizzled-7, and rmFrizzled-8 is observed, and less than 1% cross-reactivity with rmFrizzled-3 and rmFrizzled-6 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation.
Frizzled-1 is involved in the neuroprotective effect of Wnt3a against Abeta oligomers.
Fbxw7 acts as an E3 ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation.
Ramírez VT, Ramos-Fernández E, Henríquez JP, Lorenzo A, Inestrosa NC
The Journal of biological chemistry 2016 Sep 2;291(36):19092-107
The Journal of biological chemistry 2016 Sep 2;291(36):19092-107
Frizzled-1 is involved in the neuroprotective effect of Wnt3a against Abeta oligomers.
Chacón MA, Varela-Nallar L, Inestrosa NC
Journal of cellular physiology 2008 Oct;217(1):215-27
Journal of cellular physiology 2008 Oct;217(1):215-27
Fbxw7 acts as an E3 ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation.
Kanei-Ishii C, Nomura T, Takagi T, Watanabe N, Nakayama KI, Ishii S
The Journal of biological chemistry 2008 Nov 7;283(45):30540-8
The Journal of biological chemistry 2008 Nov 7;283(45):30540-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Frizzled-1 in Embryonic Mouse Intestine. Frizzled-1 was detected in immersion fixed frozen sections of embryonic mouse intestine (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1120) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.